Flow cytometric data from DAPI-stained Dinophysis acuminata (DAMV01) cells cultured in prey-saturated and prey-limited conditions over four days in March 2024

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Sung-Clarke, Serena; Ayache, Nour; Zhang, Wenguang; Smith, Juliette; Tong, Mengmeng; Brosnahan, Michael, 2025, "Flow cytometric data from DAPI-stained Dinophysis acuminata (DAMV01) cells cultured in prey-saturated and prey-limited conditions over four days in March 2024", https://doi.org/10.26027/DATAFNVBB1, MBLWHOI Library Dataverse, V1, UNF:6:A3dKuAkyNYLCjtT2Ky8Kkg== [fileUNF]
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Description
This dataset consists of a flow cytometry experiment from two cultures of Dinophysis acuminata. Cell samples were extracted from cultures that were saturated vs limited in its prey, Mesodinium rubrum, every two hours for 96 hours (4 days). Cellular DNA was stained with DAPI, which was then analyzed for particle scatter (forward, side) and fluorescence (405nm excitation, 440/50nm bandpass) on the Attune CytPix imaging flow cytometer, targeting ≥ 1000 Dinophysis events per sample. (2024-04-01)
Subject Earth and Environmental Sciences; Medicine, Health and Life Sciences; Other
Keyword dinoflagellate, Dinophysis, DNA content, replication, zygotes, sexual reproduction
Related Publication Is Cited By: Sung-Clarke, S., Ayache, N., Zhang, W., Tong, M., Smith, J. L., & Brosnahan, M. (2025). Rapid sexual reproduction in a mixotrophic dinoflagellate revealed through temporal partitioning of cellular processes. [preprint]doi: 10.1101/2025.04.24.649572
Notes Flow cytometric data and in-flow light microscopy images. The cell_cycle.atx file contains all the data including both the flow cytometric and image data and can be viewed and accessed with the Attune Cytometric software. The data from this experiment are split into three portions: the fcs, images, and stats. The .fcs datasets include flow cytometry data that includes the fluorescence and scatter values for all triggered events in each sample. The images dataset includes a .zip file containing all the images captured under the flow cytometry gating strategy. The stats dataset includes a .csv table with summary statistics for each gate in each sample.
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cell_cycle.atx
ATX Archive - 48.1 GB
Published Aug 20, 2025
12 Downloads
MD5: cf81b7d21b70862aaec8d1267de0e601
The raw Attune CytPix experiment file that can be read through the Attune cytometric software. The software provides a user interface to visualize the flow cytometry data, images, and settings together.
fcs.zip
ZIP Archive - 80.9 MB
Published Aug 20, 2025
5 Downloads
MD5: 94b3ac357673a53909cea8365eddb6b0
The flow cytometry files that include the fluorescence and scatter values for all of the triggered events. These have been compressed into a single zip archive containing all the flow cytometry files from the study.
images.zip
ZIP Archive - 48.3 GB
Published Aug 20, 2025
4 Downloads
MD5: 178e3cb05f74202eb7faa1b395eb4ee7
Images of particles that fell within gating strategy. These have been compressed into a single zip archive containing all the image files from the study.
README
Plain Text - 1.4 KB
Published Aug 20, 2025
1 Download
MD5: 2fe557bdc6f423bcda0b9e95e32f556a
ReadMe file containing descriptive information about the files in this dataset.
stats_cell_cycle_expt_3-21.tab
Tabular Data - 586.9 KB
Published Aug 20, 2025
7 Downloads
30 Variables, 2365 Observations UNF:6:A3dKuAkyNYLCjtT2Ky8Kkg==
Summary statistics of each gate in each sample
Preview "stats_cell_cycle_expt_3-21.tab"
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